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The Leica TCP SP2 AOBS confocal microscope
The division has recently purchased a Leica SP2 AOBS four laser confocal microscope system. The microscope has four lasers: a 405nm Violet Diode laser, a multi-line Blue Argon laser, a Green HeNe laser and a Far Red Diode laser. Thus the system can image four or more chromophores ranging from DAPI to CY5. Objectives range from 10x to 63x.
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The leica DM IRB IRE2 inverted microscope shown with [above] and without the Solent Scientific incubator which is used for live cell work |
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The Bio-Rad Radiance 2000 AGR-3 confocal microscope
In addition to the Leica SP2 confocal, the division has a Radiance 2000 AGR-3 three laser confocal microscope system. The microscope has three lasers: a multi-line Blue Argon laser, a Green HeNe laser and a Far Red Diode laser. Thus the system can image three more chromophores typically ranging from FITC to CY5. Objectives range from 10x to 100x.
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The Bio-Rad is attached to a Zeiss Axiovert 135 TV inverted microscope which has a Zeiss PeCon incubator fitted for live cell work and can be used with 5% carbon dioxide in air. |
| The Leica DM1L microscope |
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There is a simple inverted fluorescent microscope available for capturing images of live cells in culture or fixed specimens on slides. A dedicated Leica DC200 full colour CCD camera is controlled via DCViewer software on a Windows 98 PC.
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This microscope has 4x, 10x, 20x and 40x air objectives (some with Phase Contrast). It also has DAPI, FITC and Rhodamine wide spectra filter blocks fitted for fluorescence work.
This microscope is unsuitable for time-lapse as it has no stage incubator and the relatively cheap optics makes this microscope unsuitable for viewing specimens with low fluorescence or at high magnifications
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Bio-Rad LaserPix image analysis software (plue MS Office 2003 and PhotoShop CS) is also installed on the Leica DM1L computer. LaserPix is a Bio-Rad modified version of Image Pro Plus v4.5.
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Two Improvision 'OpenLabs' time-lapse imaging microscopes |
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| This are twoApple G4 based Improvision imaging software systems that capture images from a hi-res Hammamatsu Orca ERII B&W CCD cameras attached to a Zeiss Axiovert 100M inverted fluorescence microscopes. The systems are used for standard phase contrast and [mercury lamp] fluorescence photo-micrography. Objectives range from 10x to 100x. |
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| The OpenLabs software can be programmed for fully automatic image capture, and is ideally suited to time-lapse live cell imaging. Large in-house designed large incubators has been fitted for live cell work. Carbon dioxide can be added to the circulating incubator air. The systems have fluoresence filters for many fluorochromes ranging from DAPI to Cy5. Eppendorf 'micro-injector' systems are fitted to these microscopes. |
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| The PTI ratiometric calcium imaging system |
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This is a quantitative ratio fluorescence imaging system for measuring intracellular ion concentrations using an inverted Zeiss Avxiovert microscope and a specialsed image-intensified CCD camera. At present it has fura-2 filters fitted for intracellular calcium ion studies. |
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| PTI ImageMaster Pro software performs the ratio imaging process. ImageMaster can port ROI data to PTI's FeliX software for subsequent mathmatical analysis. |
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| The PALM MicroBeam laser micro-dissection microscope |
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| PALM [Zeiss] MicroBeam laser diistion microscope using a Hitachi colour video camera and uv laser for cutting out living cells and cells in fixed tissue sections using PALM RoboMover software. The system also has a Zeiss MRCc and MRCm camera for FITC, TRITC and CY7 fluorescence image capture via Zeiss Axioivision software. |
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Cell Biology image analysis PC workstations |
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| The division has a selection of PC's dedicated to image analysis. These IBM PC's run MetaMorph, Bio-Rad LaserPix (ImagePro Plus), Leica LCS Lite and ImageJ image analysis software. In addition they have Adobe PhotoShop and Microscoft Office installed.
There are also Apple G4 workstations running Improvision OpenLabs and Volocity, NIH Image, Microsoft Office and Adobe Photoshop software.
There are a selection of 'how to' guides available, all written in-house for these image analysis packages. Please contact Dr Keith J. Morris for further details.
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