| 3rd September | Leicester Vixens | (H) | p | Leicester Vixens Folded |
| 10th September | Worksop Town | (a) | p | Worksop in Prelim rounds of FA Cup |
| 17th September | Sparta Nottingham | (H) | ||
| 24th September | ||||
| 1st October | Chaffoteaux | (a) | ||
| 8th October | Nettleham | (H) | ||
| 15th October | Sheffield Inter | (a) | ||
| 22nd October | Nottingham Forest | (H) | ||
| 29th October | ||||
| 5th November | Leicester City | (H) | ||
| 12th November | Loughborough | (a) | ||
| 19th Novemebr | Lincoln City | (a) | ||
| 26th November | League Cup R1 | |||
| 3rd December | Belper Town | (H) | ||
| 10th December | Leicester Vixens | (a) | ||
| 17th December | Worksop Town | (H) | ||
| 2001 | ||||
| 7th January | Sparta Nottingham | (a) | ||
| 14th January | ||||
| 21st January | ||||
| 28th January | Chaffoteaux | (H) | ||
| 4th February | Nettleham | (a) | ||
| 11th February | Sheffield Inter | (H) | ||
| 18th February | ||||
| 25th February | Nottingham Forest | (a) | ||
| 4th March | Leicester City | (a) | ||
| 11th March | ||||
| 18th March | ||||
| 25th March | Loughborough | (H) | ||
| 1st April | Lincoln City | (H) | ||
| 8th April | Belper Town | (a) |