Above: Inert green fluorescent microspheres engulfed by motile lung macrophages. Lung macrophage dynamics can be quantified by flow cytometry or confocal microscopy.
Fluorescence microscopy
The Magiscan TARDIS image analysis system for the in vivo dynamic video imaging of fluorescent chemical probes within living cells
Above: Calcium transients within a bone osteoblast cell using the chemical probe FURA-2. Where blue = low Ca levels, and red = high Ca levels. Similar techniques are used to measure intra-cellular pH in lung macrophages using BCECF chemical probes.
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